Until the advent of in situ Rhodamine WT measurement systems, dye fluorometry hydrologic measurements were performed by the analysis of multiple samples physically extracted from the water body as the dye plume was naturally dispersed. Most of these investigations are performed using fluorometers designed for in vitro and pump-through measurements.
Although these methods can produce accurate hydrologic data, they are resource-intensive, significantly vulnerable to human error and other natural phenomenon. They also necessitate the field deployment of personnel throughout the duration of the study. The recent employment of in situ measurement systems has accentuated the limitations of in vitro and pump-through methods for performing these studies. As the demand for the quantity and quality of hydrologic data increases, investigators have begun to search for alternatives, and have indicated that in situ, unattended measurement will be the method of choice for future dye fluorometry hydrologic measurements.