Hach's Presence/Absence Broth (MUG) Disposable Bottles provide an easy-to-use test, ideal for screening drinking water samples for the presence of Total Coliforms and E. coli bacteria.
P/A Broth with MUG allows simultaneous detection of total coliform bacteria and E. coli. In addition to the lactose and lauryl tryptose broths with bromcresol purple, this medium contains MUG reagent (4-methylumbelliferyl-b-D-glucuronide). The MUG reagent produces a fluorogenic product when hydrolyzed by glucuronidase (an enzyme specific to E. coli). MUG detects non gasproducing (anaerogenic) strains of E. coli and works well when competitive organisms are present.
Combine 100 mL of sample and P/A Broth with MUG, incubate for 24 hours and check for a color change and fluorescence. A yellow color indicates the presence of total coliforms. To detect E. coli, examine samples under a long-wave ultraviolet (UV) light. Fluorescence indicates the presence of E. coli.
|Image||Part #||Product Description||Price||Stock||Order|
|2401612||Presence/Absence test with MUG, pack of 12||
|Usually ships in 3-5 days|
|2401650||Presence/Absence test with MUG, pack of 50||
Usually ships in 3-5 days
Can we perform an indole test instead of using UV light for confirmation?
This test is designed to use florescence as an indicator of bacteria. The indole test requires Kovac's reagent, which is not included with this item.
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Guarding the environment in Vermont
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